EDITGENE specializes in the research and development of CRISPR rapid detection technology, offering a highly sensitive, specific, and rapid isothermal nucleic acid detection method known as FASST. Leveraging our proprietary protein purification platform, we have optimized and upgraded the Cas enzyme structure, utilizing a unique crRNA design logic to produce highly efficient crRNA. Our independently designed reporter enhances detection sensitivity to the amol level and reduces detection time to just ten minutes, enabling even higher sensitivity. FASST technology allows for rapid and sensitive detection in a single-tube reaction, simplifying operational steps and reducing the risk of contamination, effectively addressing the pain points of traditional CRISPR detection methods.

Speed: Results in 5-20 minutes, truly a "rapid test".				Flexibility: Widely applicable for the detection of both DNA and RNA pathogens.
Simplicity: Single-step sampling, straightforward operation, isothermal conditions at room temperature, and portable equipment.				Accuracy: Sensitivity reaching 10 copies with strong specificity.

CRISPR Detection

---

CRISPR detection technology primarily utilizes Cas proteins such as Cas12 and Cas13. Unlike Cas9, Cas12 and Cas13 possess not only the ability to specifically cleave target sequences but also the capacity for nonspecific cleavage of other nucleic acid sequences following the cutting of the target sequence (known as the trans-cleavage activity of Cas proteins). In the activated state of trans-cleavage, they can nonspecifically cleave any single strand, shredding fluorescently labeled probes in the system, which can then be utilized for signal detection. This approach enables the development of various nucleic acid detection techniques.

FASST rapid detection technology is a high-sensitivity, high-specificity, and rapid isothermal nucleic acid detection technique based on traditional CRISPR detection technology. It relies on the synergistic action of multiple enzymes at room temperature to achieve rapid nucleic acid amplification, exhibiting dual specificity and dual signal amplification. EDITGENE provides customers with a one-stop CRISPR detection solution, offering the flexibility to choose between step-by-step customization or a comprehensive customized detection service based on your experimental needs.

▏Technical principle

● Cas Enzyme Types: LbCas12a, AapCas12b, LwaCas13a, LbuCas13a

● crRNA Design and Synthesis: We offer comprehensive services for the preparation of target gene templates, as well as the design and synthesis of isothermal amplification primers and crRNA. Place your order now

● Pricing: Custom pricing varies depending on the complexity of the project. For a free consultation, Consult with our experts at 833-2263234 or info@editxor.com

Workflow

---

Customer Provides Target Information		Preparation of Target Gene Template		Design and Synthesis of Isothermal Amplification Primers		Design and Synthesis of crRNA
Screening of crRNA Activity		Establishment and Optimization of the System		Sensitivity Testin		Specificity Evaluation

Case Study

---

● African Swine Fever Virus Detection

Utilizing the ASFV 1070 for the detection of the African Swine Fever Virus, our FASST technology is capable of detecting 1000 copies within 10 minutes and between 50 to 100 copies within 20 minutes. This demonstrates the high sensitivity and rapid response capabilities of our CRISPR-based diagnostic solution.