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New work from Jennifer Doudna’s lab could be useful for CRISPR-based technologies that seek to repurpose Cas1–Cas2 for molecular recording and information storage
Most CRISPR screens to date have been knockout screens; that is, they have relied on guide RNA–directed nucleases to introduce double-stranded breaks at targeted DNA sites. When these breaks are repaired, errors such as frameshift mutations typically result, disabling target gen…
next-generation CRISPR-based therapeutics. A nuclease discovery platform is used to broaden the targetable range of the genome, and a target-specific optimization platform is used to enable highly precise gene editing, including allele-specific editing, while maintaining high effi…
